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Related Experiment Video

Updated: May 13, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Proliferation rate of somatic cells affects reprogramming efficiency.

Yongyu Xu¹, Xiaoyuan Wei¹, Min Wang²

¹Laboratory of Receptor-based Bio-medicine, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092; National Center for Drug Screening, Stake Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

The Journal of Biological Chemistry

|February 27, 2013

Summary

This summary is machine-generated.

Removing c-Myc from the reprogramming factors (OSKM) significantly boosted induced pluripotent stem cell (iPSC) generation. Slowing somatic cell proliferation enhances iPSC induction efficiency, crucial for regenerative medicine and drug discovery.

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Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Area of Science:

Stem Cell Biology
Epigenetics
Developmental Biology

Background:

Induced pluripotent stem cells (iPSCs) offer promise for regenerative medicine and drug screening.
Current reprogramming methods using Oct-4, Sox-2, Klf-4, and c-Myc (OSKM) face limitations due to undefined mechanisms and low efficiency.
Optimizing reprogramming conditions is essential for broader iPSC applications.

Purpose of the Study:

To investigate the role of c-Myc in the OSKM reprogramming process.
To identify factors influencing the efficiency of induced pluripotent stem cell generation.
To develop improved strategies for generating iPSCs.

Main Methods:

Utilized the OSKM factor combination for reprogramming mouse embryonic fibroblasts.
Experimentally removed c-Myc from the OSKM cocktail to assess its impact.
Analyzed gene expression related to cell cycle control during reprogramming.
Applied small molecule inhibitors to modulate cell proliferation during early reprogramming stages.
Evaluated the pluripotency and developmental potential of generated iPSCs via tetraploid complementation.

Main Results:

Removing c-Myc from the OSKM combination substantially enhanced iPSC generation efficiency.
iPSCs generated without c-Myc exhibited robust pluripotency and could form full-term mice.
Forced c-Myc expression promoted early-stage fibroblast hyperproliferation, negatively correlating with reprogramming efficiency.
Inhibiting cell proliferation in early reprogramming stages improved OSKM-mediated iPSC generation.

Conclusions:

Somatic cell proliferation rate is a critical determinant of reprogramming efficiency.
Reducing proliferation of somatic cells during the initial reprogramming phase can improve iPSC induction.
The role of c-Myc in reprogramming is complex, with its proliferative effects potentially hindering pluripotency induction.