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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
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Cell separation: Terminology and practical considerations.

Matthew J Tomlinson1, Sophie Tomlinson, Xuebin B Yang

  • 1Department of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK.

Journal of Tissue Engineering
|February 27, 2013
PubMed
Summary
This summary is machine-generated.

This review clarifies cell separation terminology like purity, recovery, and viability for researchers in tissue engineering and regenerative medicine. It offers solutions to common technical challenges, improving cell sorting accuracy for all experience levels.

Keywords:
cell clusterscell separationcell sortingdead cellsterminologytissue preparation

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Area of Science:

  • Biological Sciences
  • Biotechnology
  • Regenerative Medicine

Background:

  • Cell separation is crucial in biological research, especially for tissue engineering and regenerative medicine.
  • Diverse researchers increasingly use cell separation technologies, necessitating clear guidelines.

Purpose of the Study:

  • To provide solutions for common cell sorting problems.
  • To clarify ambiguous terminology and experimental design in cell separation.
  • To offer guidance for both novice and experienced researchers.

Main Methods:

  • Review of frequently used cell separation terms: 'purity', 'recovery', and 'viability'.
  • Discussion of experimental design considerations: marker expression, tissue isolation, and cell population analysis.
  • Analysis of technical issues: cell clustering, dead cell removal, and non-specific antibody binding.

Main Results:

  • Proposes consensus definitions for key cell separation metrics.
  • Highlights the importance of robust experimental design for reliable results.
  • Offers practical solutions to common technical hurdles in cell sorting.

Conclusions:

  • Improved understanding and standardized terminology enhance cell separation quality.
  • Addressing technical challenges leads to more accurate and reproducible cell sorting.
  • This review serves as a guide to optimize cell separation techniques across various research fields.