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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 13, 2026

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Published on: July 14, 2022

Measuring cell mechanics by optical alignment compression cytometry.

Kevin B Roth¹, Charles D Eggleton, Keith B Neeves

¹Department of Chemical and Biological Engineering, Colorado School of Mines, 1600 Illinois St., Golden, CO 80401, USA.

|February 27, 2013

Summary

This summary is machine-generated.

We developed optical alignment compression (OAC) cytometry, a new method for measuring cell mechanics. This technique offers high-throughput, non-destructive analysis of individual cell mechanical properties.

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Last Updated: May 13, 2026

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

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Published on: July 14, 2022

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Area of Science:

Biophysics
Cell Biology
Microfluidics

Background:

Accurate measurement of individual cell mechanical properties is crucial for understanding cell function and disease.
Existing non-destructive techniques lack the high throughput required for comprehensive analysis.

Purpose of the Study:

To develop a novel, high-throughput, non-destructive method for measuring individual cell mechanical properties.
To introduce optical alignment compression (OAC) cytometry as a significant advancement in cell mechanics analysis.

Main Methods:

OAC cytometry utilizes hydrodynamic drag within an extensional flow microfluidic device.
Low-intensity linear optical traps align cells at the flow stagnation point.
Optical forces and hydrodynamic drag induce measurable cell deformations during cell-cell interactions.

Main Results:

OAC cytometry achieves significantly higher throughput compared to existing non-destructive methods.
The technique enables precise measurement of individual cell mechanical properties.
Deformations are induced and measured reliably through controlled cell-cell interactions.

Conclusions:

Optical alignment compression (OAC) cytometry provides a breakthrough in high-throughput, non-destructive measurement of cell mechanics.
This method enhances the ability to study cellular mechanical properties at an individual cell level.
OAC cytometry represents a significant advancement for applications in cell biology and disease diagnostics.