Ketamine effects on somatosensory cortical single neurons and on behavior in rats

The neurophysiological effects of ketamine were studied at the single-neuron level in the somatosensory cortex of unanesthetized rats behaving in a treadmill movement paradigm. Chronically implanted 25-microns microwire electrodes were used to record spontaneous discharge, sensory responses, and sensorimotor-correlated activity of single neurons before and after ketamine administration. Extracellular action potentials of up to six single neurons were simultaneously recorded for several days, allowing ketamine effects to be tested repeatedly on the same neurons. Videotaped recordings obtained during each experiment were used to measure both the sensorimotor properties of the neurons and the changes in these measures caused by different doses of ketamine. Behaviorally, ketamine produced restless-hyperactive behavior at subanesthetic doses from 5 to 20 mg/kg (intramuscularly). At higher doses (30-50 mg/kg) the rats became cataleptic and immobile after the initial hyperactive period. Whereas the spontaneous rates of most neurons were reduced or unchanged after subanesthetic doses, a subgroup (27% of the total) exhibited markedly increased firing rates. This excitation was of a tonic nature, persisting for a dose-dependent duration in a manner that was not correlated with any of the behavioral effects of the drug. In further analyses, ketamine suppressed the sensory responses of virtually all of the recorded neurons. In particular, low doses of ketamine suppressed "sensorimotor" firing (mainly proprioceptive responses) of neurons in relation to active limb movement. It also suppressed virtually all neuronal sensory responses to the sudden onset of treadmill movement, although the time-course of this effect varied from neuron to neuron. These results reveal two separable effects of ketamine: (a) a strong inhibition of all somatosensory responsiveness in this area and (b) a tonic excitatory influence expressed heterogeneously on a subgroup of neurons. This coexistence of cortical neuronal excitation and sensory suppression in the same cortical region may explain in part the mechanism of dissociative anesthesia and hallucinatory side effects observed in humans during emergence from ketamine anesthesia.