Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Epistasis01:39

Epistasis

In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
Epistasis Analysis01:09

Epistasis Analysis

Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
Genetics of Speciation02:16

Genetics of Speciation

Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Evaluation of drug-free methods for the detection of gene silencing in <i>S. cerevisiae</i>.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2025
Same author

Mutations in the DNA processivity factor POL30 predispose the FLO11 locus to epigenetic instability in S. cerevisiae.

Journal of cell science·2024
Same author

Correction: TOF1 and RRM3 reveal a link between gene silencing and the pausing of replication forks.

Current genetics·2023
Same author

Analyses of POL30 (PCNA) reveal positional effects in transient repression or bi-modal active/silent state at the sub-telomeres of S. cerevisiae.

Epigenetics & chromatin·2023
Same author

TOF1 and RRM3 reveal a link between gene silencing and the pausing of replication forks.

Current genetics·2023
Same author

Evaluation of drug-free methods for the detection of gene silencing in <i>Saccharomyces cerevisiae</i>.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2023

Related Experiment Video

Updated: May 13, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Dynamics and stability: epigenetic conversions in position effect variegation.

Krassimir Yankulov1

  • 1Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G2W1, Canada. yankulov@uoguelph.ca

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|February 28, 2013
PubMed
Summary
This summary is machine-generated.

Position effect variegation (PEV) involves stable gene expression patterns. Perturbations in dynamic histone turnover during DNA replication and transcription may drive these epigenetic conversions.

More Related Videos

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
12:52

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)
10:28

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Published on: May 5, 2023

Related Experiment Videos

Last Updated: May 13, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations
04:52

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
12:52

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)
10:28

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Published on: May 5, 2023

Area of Science:

  • Epigenetics and chromatin dynamics
  • Gene regulation in eukaryotes

Background:

  • Position effect variegation (PEV) describes stable gene expression patterns at specific genomic loci.
  • Genes under PEV can be silenced or active, with stochastic conversions causing variegated phenotypes.
  • PEV is utilized by pathogens for immune evasion and by yeasts for environmental adaptation; it likely plays a role in metazoan cell differentiation.

Purpose of the Study:

  • To revisit earlier models of PEV.
  • To review recent advances in chromatin dynamics.
  • To propose a mechanism for epigenetic conversions in PEV.

Main Methods:

  • Review of existing literature on PEV models.
  • Analysis of recent findings on chromatin dynamics.
  • Discussion of histone turnover during transcription and replication.

Main Results:

  • Earlier models of PEV are re-examined in light of new data.
  • Recent advances highlight the dynamic nature of chromatin.
  • Perturbations in histone turnover at specific sites are implicated.

Conclusions:

  • Dynamic histone turnover and its perturbations are crucial for epigenetic conversions.
  • These mechanisms are proposed to play key roles in PEV.
  • Further research into chromatin dynamics is needed to fully understand PEV.