Related Experiment Video
Updated: May 13, 2026
A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
Published on: August 22, 2025
Surface-enhanced Raman spectroscopy investigation on human breast cancer cells
Jichun Zhu1, Jing Zhou, Jianyu Guo
1State Key Laboratory of Precision Spectroscopy (East China Normal University), and Department of Physics, Shanghai 200062, P, R, China. zrsun@phy.ecnu.edu.cn.
Background:
Nanoparticles are potentially used for early cancer detection, accurate diagnosis, and cancer treatment.
Results:
In this paper, the breast cancer cells treated with gold colloidal suspension were carefully studied by surface-enhanced Raman scattering (SERS) spectra. Raman spectroscopy combining with high-resolution electron microscope is employed to investigate the interaction of gold nanoparticles (GNPs) with the intracellular components. The TEM images show that the GNPs are taken into the living cells and enveloped into some vesicles named 'lick up vesicles' in the cytosol.
Conclusions:
The SERS spectra and SERS mapping of cells indicate that the major Raman bands are mostly assigned to the vibration characteristics of proteins, and the C-H in-plane bending mode of the substituted benzene in Phenylalanine is remarkably enhanced. Finally, the interaction mechanisms of the GNPs with the intracellular components are further discussed in detail.
