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Updated: May 13, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Determination of nitrite in meat products and water using dapsone with combined spot test/diffuse reflectance on
Vitor Hugo Marques Luiz1, Leonardo Pezza, Helena Redigolo Pezza
1Instituto de Química, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Araraquara, São Paulo, Brazil.
Abstract:
This paper describes a simple, portable and environmentally friendly procedure for the determination of nitrite by combined spot test/diffuse reflectance spectroscopy on filter-paper. The method is based on the diazotization reaction of dapsone (4,4'-diamino-diphenyl sulphone, DAP) and (naphthyl)ethylenediamine hydrochloride (NED) with nitrite in acidic medium, yielding a coloured compound on the surface of a filter paper. Experimental design methodologies were used to optimise the measurement conditions. The reflectometric measurements were carried out at 545 nm and the linear range obtained was 0.29-5.0 mg L(-1) of nitrite (R=0.997), with detection and quantification limits of 0.09 and 0.29 mg L(-1), respectively. The proposed method was applied successfully to the analysis of nitrite in meat products and well water samples.
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