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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

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Related Experiment Video

Updated: May 13, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Centrobin regulates centrosome function in interphase cells by limiting pericentriolar matrix recruitment.

Jessie M Jeffery1, Ilya Grigoriev, Ina Poser

  • 1Department of Cell and Molecular Biology, Queensland Institute of Medical Research, Brisbane, QLD, Australia.

Cell Cycle (Georgetown, Tex.)
|February 28, 2013
PubMed
Summary

Centrobin regulates pericentriolar matrix levels at the centrosome. Depleting Centrobin enhances microtubule nucleation and increases pericentriolar matrix proteins, impacting microtubule organization.

Keywords:
centriole duplicationcentrobinmicrotubulesnucleationpericentriolar matrix

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Last Updated: May 13, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • The pericentriolar matrix (PCM) is crucial for microtubule nucleation and centriole duplication.
  • Mechanisms regulating PCM levels at the centrosome remain incompletely understood.

Purpose of the Study:

  • To investigate the role of the centrosomal protein Centrobin in regulating pericentriolar matrix levels.
  • To elucidate Centrobin's contribution to microtubule nucleation and organization.

Main Methods:

  • Depletion of Centrobin in cells using specific techniques.
  • Analysis of interphase microtubule arrays and stability.
  • Assessment of microtubule nucleation rates and dynamics at the centrosome.
  • Quantification of pericentriolar matrix protein recruitment (e.g., γ-tubulin, AKAP450, Kendrin, PCM-1).

Main Results:

  • Centrobin depletion leads to more focused and less stable interphase microtubule arrays.
  • Cells lacking Centrobin exhibit accelerated microtubule nucleation with increased growing microtubule ends.
  • Microtubule plus end dynamics parameters remain largely unchanged upon Centrobin depletion.
  • Increased recruitment of key PCM proteins, including γ-tubulin, AKAP450, Kendrin, and PCM-1, to the centrosome was observed.

Conclusions:

  • Centrobin plays a significant role in controlling the amount of pericentriolar matrix at the centrosome.
  • Centrobin's regulation of PCM levels influences microtubule nucleation and overall microtubule organization within the cell.