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Published on: April 15, 2014
Ciliary neurotrophic factor role in myelin oligodendrocyte glycoprotein expression in Cuprizone-induced multiple
Zivar Salehi1, Sara Pishgah Hadiyan, Reza Navidi
1Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. geneticzs@yahoo.co.uk
Abstract:
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system that leads to loss of myelin and oligodendrocytes and damage to axons. Myelin oligodendrocyte glycoprotein (MOG) is a minor component of the myelin sheath, but is an important autoantigen linked to the pathogenesis of MS. Ciliary neurotrophic factor (CNTF) has been shown to enhance the generation, maturation, and survival of oligodendrocytes in culture medium. The aim of this study was to demonstrate the role of CNTF on MOG expression in the cerebral cortex of Cuprizone-induced MS mice. The mice were treated by Cuprizone for five weeks in order to induce MS. The mice were then divided into 3 groups. The first group was injected subcutaneously (SC) by CNTF in the amount of 250 μg/kg BW per day. The second group (SHAM) was injected SC by normal saline and the third group was left without injection as the control group. After four weeks the mice were killed and the cerebral cortex was harvested and the expression of MOG was studied by Western blotting. The data from this study show that the MOG expression was significantly increased in the CNTF-injected group as compared to the other groups. It is concluded that CNTF increases the MOG expression and may be important in the pathophysiology of MS. It is also concluded that CNTF may play a role in the process of remyelination by inducing the MOG expression.
Insights
Ciliary neurotrophic factor (CNTF) significantly increased myelin oligodendrocyte glycoprotein (MOG) expression in a mouse model of multiple sclerosis (MS). This suggests CNTF may play a role in MS pathophysiology and remyelination.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Multiple sclerosis (MS) is a central nervous system inflammatory disease causing myelin and oligodendrocyte loss, and axonal damage.
- Myelin oligodendrocyte glycoprotein (MOG) is a key autoantigen in MS pathogenesis.
- Ciliary neurotrophic factor (CNTF) promotes oligodendrocyte generation, maturation, and survival in vitro.
Purpose of the Study:
- To investigate the effect of CNTF on MOG expression in the cerebral cortex of Cuprizone-induced MS mice.
- To explore the potential role of CNTF in MS pathophysiology and remyelination.
Main Methods:
- Multiple sclerosis was induced in mice using Cuprizone for five weeks.
- Mice were divided into three groups: CNTF injection (250 μg/kg BW/day), saline injection (SHAM), and control (no injection).
- Cerebral cortex MOG expression was analyzed using Western blotting after four weeks of treatment.
Main Results:
- CNTF administration significantly increased MOG expression in the cerebral cortex compared to SHAM and control groups.
- The data indicate a direct correlation between CNTF treatment and elevated MOG levels.
Conclusions:
- CNTF administration enhances MOG expression in a mouse model of MS.
- CNTF may be implicated in the pathophysiology of MS by modulating MOG expression.
- CNTF could play a role in remyelination processes through the induction of MOG expression.
