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Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography
Published on: April 19, 2016
The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat (Rattus norvegicus)
Sandro Wolf1, Jochen Reetz, Reimar Johne
1Institute for Microbiology, Technische Universität Dresden, Dresden, Germany. sandro.wolf@tu-dresden.de
Abstract:
Wild rats can be reservoirs and vectors for several human pathogens. An initial RT-PCR screening of the intestinal contents of Norway rats trapped in the sewer system of Copenhagen, Denmark, for caliciviruses revealed the presence of a human norovirus in one of 11 rodents. Subsequent phylogenetic analysis of the ~4.0-kb 3'-terminus of the norovirus genome resulted in the identification of a recombinant GI.b/GI.6 strain. The simultaneous detection of hepatitis E virus-like particles in the feces of this rat by transmission electron microscopy was confirmed by RT-PCR and sequence determination, resulting in the identification of a novel rat hepatitis E virus.
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