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Related Experiment Video

Updated: May 13, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

A toolbox for generating single-stranded DNA in optical tweezers experiments.

Andrea Candelli1, Tjalle P Hoekstra, Geraldine Farge

  • 1Institute for Lasers, Life and Biophotonics, Department of Physics and Astronomy, VU University Amsterdam, Amsterdam, 1081, HV, The Netherlands.

Biopolymers
|February 28, 2013
PubMed
Summary

Researchers developed new DNA templates for single-molecule studies. These templates allow the creation of long single-stranded DNA (ssDNA) segments, crucial for understanding DNA repair and replication processes.

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Genomics

Background:

  • DNA replication and repair involve single-stranded DNA (ssDNA) or specific DNA junctions.
  • Studying DNA-protein interactions using optical trapping requires specialized DNA templates, particularly for ssDNA.
  • Existing methods for generating ssDNA constructs for optical tweezers are limited.

Purpose of the Study:

  • To present novel DNA templates for generating kilobase-length ssDNA segments.
  • To enable single-molecule studies of DNA-protein interactions on ssDNA.
  • To investigate and optimize the production of ssDNA for biophysical experiments.

Main Methods:

  • Biotinylated double-stranded DNA (dsDNA) templates were tethered between optically trapped microspheres.
  • Force-induced DNA melting was used to convert dsDNA into partial or complete ssDNA molecules.

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Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
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Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Related Experiment Videos

Last Updated: May 13, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers
10:08

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

  • Systematic investigation of force-induced melting kinetics, ionic strength effects, DNA sequence/length impacts, and microsphere size effects on ssDNA tether stability.

    • Main Results:

    • Demonstrated the generation of kilobase-length ssDNA segments using force-induced DNA melting.
    • Quantified the influence of ionic strength, DNA characteristics, and microsphere size on ssDNA tether formation and stability.
    • Provided insights into the variables affecting ssDNA production for single-molecule studies.

    Conclusions:

    • Developed versatile DNA templates for creating long ssDNA segments suitable for optical tweezers experiments.
    • Established a foundation for optimizing DNA template design for investigating protein interactions on ssDNA.
    • Advanced the capability to study DNA-protein binding kinetics and mechanisms on ssDNA using single-molecule techniques.