Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Structure

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The RANK/RANKL axis controls vascular dynamics in the bone marrow.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Red Blood Cell-Mediated Enhancement of Hematopoietic Stem Cell Functions via a Hes1-Dependent Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Same author

The interpretable surgical temporal informer: explainable surgical time completion prediction.

International journal of computer assisted radiology and surgery·2025

Same author

Automated Prediction of Bone Volume Removed in Mastoidectomy.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2025

Same author

Pyruvate-GPR31 axis induces LysoDC dendrite protrusion to M-cell pockets for effective immune responses.

Gut microbes·2025

Same author

Central compartment of nasal cavity-derived MMP-9 enhances mixed-type 2 inflammation in eosinophilic chronic rhinosinusitis.

International immunology·2025

Search research articles

Related Experiment Video

Updated: May 13, 2026

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Published on: February 24, 2023

[Analysis of bone tissues by using fluorescent imaging].

Junichi Kikuta¹, Masaru Ishii

¹Osaka University, Japan Science and Technology, CREST, Japan.

Clinical Calcium

|March 1, 2013

Summary

This summary is machine-generated.

Advanced live bone imaging reveals how RANKL controls osteoclast function in vivo. This study visualizes mature osteoclasts and their bone resorption activity using multiphoton microscopy.

More Related Videos

Analysis and Imaging of Osteocytes

Analysis and Imaging of Osteocytes

Published on: November 29, 2024

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

Related Experiment Videos

Last Updated: May 13, 2026

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Published on: February 24, 2023

Analysis and Imaging of Osteocytes

Analysis and Imaging of Osteocytes

Published on: November 29, 2024

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

Area of Science:

Biomedical Engineering
Cell Biology
Skeletal Biology

Context:

* Understanding cellular dynamics in living organisms is crucial for biological and medical research.
* Visualizing bone tissue at the cellular level in vivo presents significant technical challenges.
* Osteoclasts play a key role in bone remodeling and diseases like osteoporosis.

Purpose:

* To establish and detail an advanced imaging system for visualizing live bone tissues using intravital multiphoton microscopy.
* To visualize mature osteoclasts in live bone and investigate their function in vivo.
* To develop novel tools for analyzing osteoclast behavior and detecting bone resorption.

Summary:

* An advanced intravital multiphoton microscopy system was developed for live bone tissue imaging.
* Mature osteoclasts were visualized in live bone, demonstrating that RANKL regulates their bone-resorptive functions.
* New image analysis software and pH-sensing fluorescent probes were created to track osteoclast morphology and detect bone resorption.
* The study presents the latest data, detailed methodology, and discusses future applications of this intravital imaging technique.

Impact:

* Provides unprecedented insights into the dynamic behavior of osteoclasts in their native bone microenvironment.
* Offers a powerful platform for studying bone remodeling, diseases, and therapeutic interventions.
* Enables precise detection and monitoring of bone resorption at the tissue surface.