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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 13, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Reflection-mode multifocal optical-resolution photoacoustic microscopy.

Guo Li, Konstantin I Maslov, Lihong V Wang

    Journal of Biomedical Optics
    |March 1, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Multifocal optical-resolution photoacoustic microscopy (OR-PAM) significantly boosts imaging speed by using multiple light foci and an ultrasonic array. This advanced OR-PAM system enables rapid in vivo imaging of microvessels with high resolution.

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    Last Updated: May 13, 2026

    Three-dimensional Optical-resolution Photoacoustic Microscopy
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    Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
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    Published on: June 26, 2017

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
    11:21

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

    Published on: January 15, 2013

    Area of Science:

    • Biomedical Optics
    • Photoacoustic Imaging
    • Microscopy

    Background:

    • Single-focus optical-resolution photoacoustic microscopy (OR-PAM) has limitations in imaging speed.
    • Multifocal OR-PAM offers a potential solution by employing multifocal optical illumination and an ultrasonic array transducer.

    Discussion:

    • A reflection-mode multifocal OR-PAM system was developed using a microlens array for multifocal illumination and an ultrasonic array for simultaneous wave detection.
    • A microprism was utilized to align the multiple optical foci confocally with the ultrasonic transducer's focal zone, optimizing signal acquisition.

    Key Insights:

    • The developed multifocal OR-PAM system demonstrates capability for in vivo microvessel imaging.
    • It achieves imaging of a 6×5×2.5 mm³ volume with 16 μm lateral resolution in approximately 2.5 minutes.
    • Imaging speed was primarily limited by the signal multiplexing ratio and laser pulse repetition rate.

    Outlook:

    • Further optimization of signal processing and laser parameters could enhance imaging speed.
    • This technology holds promise for faster and more efficient in vivo microscopic imaging applications.
    • Potential for broader applications in biomedical research and clinical diagnostics.