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Updated: May 13, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

A diamond nanoneedle array for potential high-throughput intracellular delivery.

Xianfeng Chen¹, Guangyu Zhu, Yang Yang

¹Center of Super-Diamond and Advanced Films (COSDAF) and Department of Physics and Materials Science, City University of Hong Kong, Hong Kong SAR, P. R. China. xianfeng.chen@cityu.edu.hk.

Advanced Healthcare Materials

|March 1, 2013

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A dense diamond nanoneedle array enables rapid, high-throughput cellular delivery of molecules like drugs and fluorescent probes. This method bypasses cellular uptake mechanisms such as endocytosis for efficient delivery.

Area of Science:

Biotechnology
Nanotechnology
Cell Biology

Background:

Efficient intracellular delivery of molecules is crucial for research and therapeutics.
Traditional methods like endocytosis can be slow and inefficient for certain applications.

Purpose of the Study:

To develop a novel, high-throughput method for delivering molecules into cells.
To utilize diamond nanoneedles for direct intracellular cargo delivery.

Main Methods:

Fabrication of a dense array of diamond nanoneedles.
Application of the nanoneedle array for direct delivery of fluorescent probes and drug molecules to cells.
Assessment of delivery efficiency and comparison with endocytosis-dependent methods.

Main Results:

Keywords:

drug delivery fluorescent cell probes high-throughput intracellular delivery nanoneedle arrays

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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

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The diamond nanoneedle array demonstrated rapid and convenient delivery of molecules to a large number of cells.
Successful delivery of fluorescent probes and drug molecules was achieved.
The delivery mechanism was shown to bypass the need for endocytosis.

Conclusions:

Dense diamond nanoneedle arrays offer a promising platform for high-throughput intracellular delivery.
This technology has potential applications in drug delivery, gene therapy, and cellular imaging.
The method provides an alternative to endocytosis for direct molecular delivery into cells.