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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 13, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Identification of radiation-induced microRNA transcriptome by next-generation massively parallel sequencing.

M Ahmad Chaudhry1, Romaica A Omaruddin, Christopher D Brumbaugh

  • 1Department of Medical Laboratory and Radiation Sciences, University of Vermont, Burlington, VT 05405, USA.

Journal of Radiation Research
|March 1, 2013
PubMed
Summary
This summary is machine-generated.

Ionizing radiation (IR) alters micro-RNA (miRNA) expression in human cells, impacting gene regulation. This study identifies specific miRNA changes over time following IR exposure, providing insights into cellular stress responses.

Keywords:
TK6 cellsdifferential gene expressionmicro-RNAnext-generation sequencingradiation effects

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Area of Science:

  • Molecular Biology
  • Genetics
  • Radiation Biology

Background:

  • Gene regulation in cells exposed to ionizing radiation (IR) occurs at transcriptional and post-transcriptional levels.
  • Micro-RNAs (miRNAs) are key regulators of post-transcriptional gene expression.
  • Previous studies indicated altered miRNA expression in IR-treated cells.

Purpose of the Study:

  • To comprehensively analyze genome-wide miRNA transcriptome changes in human cells following X-ray irradiation.
  • To identify specific miRNAs and their temporal expression patterns in response to radiation exposure.

Main Methods:

  • Massively parallel sequencing of miRNA transcriptome in human cells.
  • Irradiation of cells with X-rays.
  • Application of the Short Time-series Expression Miner (STEM) tool for temporal expression profiling.

Main Results:

  • Baseline expression analysis revealed modulation of 402 miRNAs without IR.
  • 190 miRNAs were clustered into six significant temporal expression profiles post-IR.
  • Specific miRNAs like miR-19b and miR-93 were induced, while miR-222, miR-92a, and miR-941 were repressed.
  • Multiple miRNAs exhibited dual induction peaks at 8 and 24 hours post-irradiation.
  • Several miRNAs showed induction specifically at the 8-hour time-point.

Conclusions:

  • This study provides a detailed catalogue of miRNA modulation in response to radiation.
  • The identified miRNA expression profiles offer insights into the mechanisms of gene regulation under cellular stress.
  • These findings contribute to understanding the cellular response to ionizing radiation.