Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 13, 2026

Mechanical Vessel Injury in Zebrafish Embryos
06:38

Mechanical Vessel Injury in Zebrafish Embryos

Published on: February 17, 2015

Chemically induced intestinal damage models in zebrafish larvae.

Stefan H Oehlers1, Maria Vega Flores, Christopher J Hall

  • 1Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New Zealand.

Zebrafish
|March 2, 2013
PubMed
Summary

This study details two zebrafish models for intestinal damage, using dextran sodium sulfate (DSS) and trinitrobenzene sulfonic acid (TNBS), to mimic human inflammatory bowel disease (IBD) and explore host responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide association study reveals a novel tuberculosis susceptibility locus in multiple East Asian and European populations.

Genome medicine·2026
Same author

Loss of 7-Dehydrocholesterol Reductase mediated cholesterol biosynthesis activates IRF3 and inhibits control of <i>Mycobacterium marinum</i> infection.

bioRxiv : the preprint server for biology·2026
Same author

A Cationic Supramolecule With Potent Antifungal Activity, Single-Species Selectivity, and Strong Synergy With Echinocandins.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A bioluminescence-based chemical screen identifies a bactericidal naphthalen-1-ylmethanamine scaffold targeting MmpL3 in Mycobacterium abscessus.

Cell reports·2026
Same author

A transgenic zebrafish leukemia model driven by KMT2A::MLLT3 (MLL-AF9).

Leukemia·2026
Same author

Smooth-to-rough morphotype switching, a mechanism of phage resistance in <i><i>Mycobacterium</i> abscessus</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

Area of Science:

  • Zebrafish models for disease research
  • Gastrointestinal research
  • Inflammatory bowel disease (IBD) modeling

Background:

  • Zebrafish are increasingly used to model human diseases, including inflammatory bowel disease (IBD).
  • Existing zebrafish intestinal damage models often involve immersion in colitogenic agents.
  • Standardized chemical induction methods are needed for reproducible IBD modeling in zebrafish.

Purpose of the Study:

  • To describe methods for generating two chemically induced zebrafish intestinal damage models.
  • To detail techniques for monitoring inflammation and intestinal damage in these models.
  • To present chemical-genetic approaches for manipulating host responses in zebrafish IBD models.

Main Methods:

  • Induction of intestinal damage using dextran sodium sulfate (DSS) in zebrafish.

More Related Videos

Microgavage of Zebrafish Larvae
10:53

Microgavage of Zebrafish Larvae

Published on: February 20, 2013

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors
04:53

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Published on: August 25, 2022

Related Experiment Videos

Last Updated: May 13, 2026

Mechanical Vessel Injury in Zebrafish Embryos
06:38

Mechanical Vessel Injury in Zebrafish Embryos

Published on: February 17, 2015

Microgavage of Zebrafish Larvae
10:53

Microgavage of Zebrafish Larvae

Published on: February 20, 2013

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors
04:53

Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors

Published on: August 25, 2022

  • Induction of intestinal damage using trinitrobenzene sulfonic acid (TNBS) in zebrafish.
  • Monitoring intestinal damage and inflammatory markers.
  • Application of chemical-genetic tools to modulate host response.

    • Main Results:

    • Established reproducible methods for DSS- and TNBS-induced intestinal damage in zebrafish.
    • Characterized the inflammatory processes and damage severity in both models.
    • Demonstrated the utility of chemical-genetic manipulation for studying host-pathogen interactions.

    Conclusions:

    • Chemically induced zebrafish models using DSS and TNBS provide valuable tools for IBD research.
    • These models allow for the study of intestinal inflammation and host responses.
    • Further research can utilize these models to investigate therapeutic strategies for IBD.