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Updated: May 13, 2026

Swine Model of Biofilm Infection and Invisible Wounds
Published on: June 16, 2023
[Wound microbial sampling methods in surgical practice, imprint techniques]
Z Chovanec1, L Veverková, M Votava
11. chirurgická klinika LF MU a Fakultní nemocnice u svaté Anny v Brně. zdenek.chovanec@fnusa.cz
Abstract:
The wound is a damage of tissue. The process of healing is influenced by many systemic and local factors. The most crucial and the most discussed local factor of wound healing is infection. Surgical site infection in the wound is caused by micro-organisms. This information is known for many years, however the conditions leading to an infection occurrence have not been sufficiently described yet. Correct sampling technique, correct storage, transportation, evaluation, and valid interpretation of these data are very important in clinical practice. There are many methods for microbiological sampling, but the best one has not been yet identified and validated. We aim to discuss the problem with the focus on the imprint technique.
Insights
Surgical site infections impede wound healing. This study examines microbiological sampling techniques, focusing on the imprint method to improve infection detection and patient outcomes.
Area of Science:
- Wound healing and tissue repair processes.
- Microbiology and infectious diseases.
- Clinical diagnostics and laboratory medicine.
Context:
- Wound healing is a complex process influenced by various factors.
- Infection is a critical local factor significantly impacting wound healing.
- Surgical site infections (SSIs) are a common complication caused by microorganisms.
Purpose:
- To discuss the challenges in identifying and managing wound infections.
- To highlight the importance of accurate microbiological sampling in clinical practice.
- To evaluate the imprint technique as a potential method for microbiological wound sampling.
Summary:
- Microbiological sampling, storage, transportation, and interpretation are crucial for effective wound infection management.
- Current methods for microbiological wound sampling lack universal validation, necessitating further research.
- The imprint technique is proposed as a focus for discussion due to its potential advantages in wound sampling.
Impact:
- Improved diagnostic accuracy for wound infections.
- Enhanced clinical decision-making regarding infection treatment.
- Potential for optimized wound care protocols and better patient recovery.
- Advancement in the validation of microbiological sampling techniques.

