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Related Experiment Video

Updated: May 13, 2026

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

Human eosinophils express functional CCR7.

Praveen Akuthota¹, Shigeharu Ueki, Jessica Estanislau

¹Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. pakuthot@bidmc.harvard.edu

American Journal of Respiratory Cell and Molecular Biology

|March 2, 2013

Summary

This summary is machine-generated.

Human eosinophils express the CCR7 receptor, enabling them to respond to chemokines like CCL19 and CCL21. This finding is crucial for understanding eosinophil migration in allergic inflammation.

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Last Updated: May 13, 2026

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Published on: May 30, 2020

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Detection of True IgE-expressing Mouse B Lineage Cells

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Published on: December 1, 2014

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Human eosinophils are key players in allergic inflammation.
Eosinophil migration to lymph nodes is observed, but the underlying mechanisms are unclear.
CCR7 is a critical chemokine receptor for leukocyte trafficking to lymph nodes.

Purpose of the Study:

To investigate CCR7 expression in human eosinophils.
To determine functional responses of eosinophils to CCR7 ligands CCL19 and CCL21.

Main Methods:

Purification of human eosinophils from healthy donors.
Assessment of CCR7 expression using flow cytometry and Western blot.
Chemotaxis, shape change, calcium flux, and ERK phosphorylation assays.

Main Results:

Human eosinophils express CCR7 on their surface.
IL-5-primed eosinophils show dose-dependent chemotaxis to CCL19 and CCL21.
Eosinophils exhibit shape changes, calcium influxes, and ERK phosphorylation upon stimulation with CCL19/CCL21.

Conclusions:

Human eosinophils express functional CCR7.
CCR7 mediates eosinophil responses to CCL19 and CCL21, including migration and intracellular signaling.
This study elucidates a novel mechanism for eosinophil trafficking in inflammatory conditions.