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An ELISA for quantitation of tetanus toxin
A Pal1, R Kumar, B L Jailkhani
1Department of Microbiology, All India Institute of Medical Sciences, New Delhi.
The Indian Journal of Medical Research
|March 1, 1990
Abstract:
A sandwich ELISA for quantitation of tetanus toxin was devised using commercially available horse antitetanus antiserum and its HRP-conjugate. With commercial toxoid as a standard the assay had a lower sensitivity of 100 ng/ml and was linear up to 100 micrograms/ml. In terms of Lf equivalents the ELISA assay was linear from 0.02 to 20 Lf/ml. The Lf activity in 20 preparations of tetanus toxin as determined by ELISA was comparable to the activity measured by conventional flocculation tests (r = 0.987).