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Published on: December 3, 2019
Drug absorption efficiency in Caenorhbditis elegans delivered by different methods
Shan-Qing Zheng1, Ai-Jun Ding, Guo-Ping Li
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.
Plos One
|March 2, 2013
Summary
Drug absorption in Caenorhabditis elegans (C. elegans) varies by method. The NGM dead and liquid growing methods show the best efficiency for compounds like resveratrol and 5-Fluoro-2'-deoxyuridine (FUDR).
Area of Science:
- Biomedical Research
- Model Organisms
- Pharmacology
Background:
- Caenorhabditis elegans is a widely used model organism in various research fields.
- Drug administration methods in C. elegans studies are inconsistent, hindering result comparison.
Purpose of the Study:
- To evaluate and compare the drug absorption efficiency of five common methods in C. elegans.
- To assess the absorption and metabolism patterns of resveratrol and 5-Fluoro-2 -deoxyuridine (FUDR) using these methods.
Main Methods:
- Five drug delivery methods were tested: LB medium, NGM with live bacteria, NGM with dead bacteria, spot dead method, and liquid growing method.
- Resveratrol (low aqueous solubility) and FUDR (water-soluble) were used as test compounds.
- Drug concentrations in C. elegans and medium were measured over time.
Main Results:
- Drug concentrations in C. elegans peaked between 12 hours and one day, then slowly decreased.
- Higher drug concentrations correlated with higher metabolism rates.
- Absorption was more efficient with dead bacteria compared to live bacteria.
- NGM dead method and liquid growing method demonstrated the highest absorption efficiency.
Conclusions:
- Resveratrol and FUDR exhibit similar absorption and metabolism patterns in C. elegans.
- Drug metabolism is dependent on both dosage and time.
- The NGM dead and liquid growing methods are superior for drug absorption in C. elegans.
- Worm drug concentrations are comparable to mouse levels, aiding dose translation.
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