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Evaluation of cyanocobalamin in multivitamin tablets and their Standard Reference Material 3280 by HPLC with visible
Teruki Matsumoto1, Jun Takebayashi, Yoshiko Ishimi
1National Institute of Health and Nutrition, Department of Food Function and Labeling, 1-23-1, Toyama, Shinjuku, Tokyo 162-8636, Japan. teruki@nih.go.jp
Abstract:
As a part of the development of an alternative to microbiological assay for vitamin B12, we performed a quantitative analysis of cyanocobalamin (CN-cbl) in a National Institute of Standards and Technology Standard Reference Material (SRM 3280) by HPLC. Using this method, the observed value (4.64 microg/g) of CN-cbl in SRM 3280 was found to be in good agreement with the certified value (4.80 microg/g). The accuracy was over 95%, with a corresponding measurement precision value of 5%. To evaluate the applicability of the method on commercial multivitamin tablets, the method was applied to a variety of these samples. The present method has a good accuracy and precision to evaluate CN-cbl with respect to all of the examined tablets.
