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Performance of the AOAC use-dilution method with targeted modifications: collaborative study.

Stephen F Tomasino¹, Albert E Parker, Martin A Hamilton

¹U.S. Environmental Protection Agency, Office of Pesticide Programs, Biological and Economic Analysis Division, Microbiology Laboratory Branch, Environmental Science Center, Fort Meade, MD 20755-5350, USA. Tomasino.Stephen@epamail.epa.gov

Journal of AOAC International

|March 5, 2013

Summary

This summary is machine-generated.

This study enhanced the AOAC use-dilution method (UDM) for disinfectant testing. While major modifications were not adopted due to increased variability, a key step for preparing Pseudomonas aeruginosa test cultures was recommended for adoption.

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Area of Science:

Microbiology
Environmental Science
Analytical Chemistry

Background:

The AOAC use-dilution method (UDM) is a standard for evaluating disinfectant efficacy.
Laboratories using the UDM identified challenges, particularly with Pseudomonas aeruginosa culture preparation.
A collaborative study was initiated by the U.S. Environmental Protection Agency (EPA) to address these issues.

Purpose of the Study:

To evaluate modifications aimed at improving the AOAC use-dilution method (UDM).
Specifically, to enhance the preparation of test cultures, focusing on Pseudomonas aeruginosa.
To determine if proposed modifications reduce method variability and improve reliability.

Main Methods:

A five-laboratory trial compared a modified UDM against the current UDM.
Modifications included an agar-based approach for growing test cultures.
Efficacy was assessed using three variables: positive carriers, log reduction, and pass/fail outcome, with specific disinfectant and soil load conditions.

Main Results:

The major modification (agar-based culture) did not reduce method variability; repeatability and reproducibility were not improved.
The modified method's variability was equal to or greater than the current UDM.
However, the frozen stock preparation step of the modified method showed statistical equivalency and was proposed for adoption.

Conclusions:

The proposed agar-based modification for test culture preparation was not supported due to increased variability.
The frozen stock preparation step from the modified UDM is recommended for procedural adoption.
The study provided valuable data on UDM variability and suggests a reassessment of its performance standards.