Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering carotenoid and steroidal glycoalkaloid depleted tomato fruit for heterologous production of high value terpenes.

bioRxiv : the preprint server for biology·2026
Same author

BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana.

BMC plant biology·2026
Same author

Development of male-sterile lines of Setaria viridis to accelerate C<sub>4</sub> model plant genetics.

The Plant journal : for cell and molecular biology·2026
Same author

Virus-mediated, heritable gene editing in groundcherry (<i>Physalis grisea</i>).

Frontiers in plant science·2026
Same author

Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.

The Plant journal : for cell and molecular biology·2026
Same author

Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.).

Plant biotechnology journal·2025
Same journal

Long-Range Signals Built upon Plant Structural Continuity.

Annual review of plant biology·2026
Same journal

The Power of Symbiosis in Life and Science.

Annual review of plant biology·2026
Same journal

RNA Meets Agriculture: From Molecular Mechanisms to Market Applications.

Annual review of plant biology·2026
Same journal

Sensing Plant Photosynthesis Using Solar-Induced Chlorophyll Fluorescence: From Chloroplasts to the Globe.

Annual review of plant biology·2026
Same journal

The Structure and Function of the Chloroplast Import Apparatus.

Annual review of plant biology·2026
Same journal

A Multidimensional View of Biomolecular Condensates in Plant Biology.

Annual review of plant biology·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Plant genome engineering with sequence-specific nucleases.

Daniel F Voytas1

  • 1Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA. voytas@umn.edu

Annual Review of Plant Biology
|March 5, 2013
PubMed
Summary
This summary is machine-generated.

Genome engineering allows scientists to precisely alter plant DNA, enabling new research and agricultural applications. This technology facilitates targeted gene modifications for improved crop production and understanding plant biology.

More Related Videos

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

Related Experiment Videos

Last Updated: May 13, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

Area of Science:

  • Plant Biology
  • Genetics
  • Biotechnology

Background:

  • Recent breakthroughs in genome engineering offer unprecedented control over plant genetic material.
  • Scientists can now modify DNA in living plant cells with high precision.

Purpose of the Study:

  • To detail the capabilities of modern genome engineering techniques in plants.
  • To highlight the potential of these technologies for basic research and agricultural applications.

Main Methods:

  • Utilizing sequence-specific nucleases to create targeted double-strand DNA breaks.
  • Employing DNA repair mechanisms (homologous recombination or nonhomologous end joining) to achieve desired sequence modifications.
  • Introducing specific nucleotide substitutions, deleting genes/chromosomal segments, or inserting foreign DNA at precise locations.

Main Results:

  • Demonstrated ability to precisely alter plant DNA sequences.
  • Enabled targeted modifications including gene alterations and insertions.
  • Established a controllable method for DNA repair to achieve specific outcomes.

Conclusions:

  • Genome engineering significantly advances fundamental plant research by connecting DNA sequences to biological functions.
  • This technology holds immense potential for enhancing the biosynthetic capabilities of plants to meet global agricultural demands.