Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Factors02:16

Transcription Factors

70.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
70.9K
Histone Modification02:32

Histone Modification

14.7K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.7K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
General Transcription Factors01:30

General Transcription Factors

5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular mechanisms of the MLL4 complex in H3K4 methylation and p53-dependent transcription activation.

Molecular cell·2026
Same author

HIV-1 uncoating location dictates sites of integration.

Nature communications·2026
Same author

Regulation of B cell development and lymphocyte function by transcriptional coactivator OCA-B.

Frontiers in immunology·2026
Same author

Recruitment of BRD4 to the ASXL1 genomic targets depends on the extra-terminal domain of BRD4.

Nature communications·2026
Same author

H4K16 acylations destabilize chromatin architecture and facilitate transcriptional response during metabolic perturbations.

Molecular cell·2025
Same author

MED1 IDR deacetylation controls stress responsive genes through RNA Pol II recruitment.

Nature chemical biology·2025

Related Experiment Video

Updated: May 6, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K

H3K4me3 interactions with TAF3 regulate preinitiation complex assembly and selective gene activation.

Shannon M Lauberth1, Takahiro Nakayama, Xiaolin Wu

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.

Cell
|March 5, 2013
PubMed
Summary

Histone mark H3K4me3 guides transcription factor TFIID to active genes, enhancing p53-driven gene expression. This mechanism ensures rapid p53 target gene induction during genotoxic stress.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.1K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

7.7K

Related Experiment Videos

Last Updated: May 6, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.1K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

7.7K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Histone modifications control chromatin-based processes, but their specific roles are not fully understood.
  • Histone H3 trimethylated at lysine 4 (H3K4me3) is linked to active genes and aids transcription via effector proteins like TFIID.

Purpose of the Study:

  • To elucidate the mechanism by which H3K4me3 influences gene transcription, particularly in the context of p53 target genes.
  • To investigate the role of H3K4me3-TAF3 interactions in directing TFIID recruitment and preinitiation complex formation.

Main Methods:

  • Investigated interactions between H3K4me3 and TAF3, a component of TFIID.
  • Analyzed the impact of H3K4me3 on p53-dependent transcription and preinitiation complex (PIC) formation.
  • Examined the interplay between H3K4me3, TAF3, TATA box, and PIC assembly in gene regulation.

Main Results:

  • H3K4me3-TAF3 interactions are crucial for global TFIID recruitment to active genes, including p53 targets.
  • H3K4me3 enhances p53-dependent transcription by promoting PIC formation, acting independently or with the TATA box.
  • H3K4me3-TAF3/TFIID interactions modulate p53's gene-selective functions in response to genotoxic stress.

Conclusions:

  • H3K4me3 serves as a key regulator, directing PIC assembly through TAF3/TFIID interactions.
  • This mechanism facilitates the rapid induction of specific p53 target genes upon genotoxic stress.
  • The study reveals a novel pathway for epigenetic control of gene transcription and cellular response.