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Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
Published on: May 10, 2021
Valvular osteoclasts in calcification and aortic valve stenosis severity
Edit Nagy1, Per Eriksson, Mohamed Yousry
1Department of Medicine, Karolinska Institutet, Stockholm, Sweden; Department of Cardiology, Karolinska University Hospital, Stockholm, Sweden.
International Journal of Cardiology
|March 5, 2013
Summary
Bone turnover mediators, including TRAP and RANK ligand, correlate with aortic stenosis severity. Local bone turnover markers in calcified valves predict stenosis progression, highlighting their critical role.
Area of Science:
- Cardiovascular Biology
- Bone Metabolism
- Aortic Valve Disease
Background:
- Aortic valve calcification involves bone remodeling, likely initiated by microfractures and cellular recruitment.
- Understanding the mediators and spatial distribution of bone turnover is crucial for assessing disease progression.
Purpose of the Study:
- To evaluate circulating bone homeostasis mediators in patients with aortic stenosis.
- To correlate these mediators with the severity of aortic stenosis.
- To investigate the spatio-temporal distribution of bone turnover within calcified aortic valve tissue.
Main Methods:
- Analysis of plasma and explanted aortic valves from 46 patients undergoing aortic valve replacement.
- Measurement of plasma levels of tartrate-resistant acid phosphatase (TRAP), RANK ligand, and Runx2/Cbfa1.
- Quantitative real-time PCR to assess mRNA levels of bone turnover markers in different valve regions.
Main Results:
- Plasma TRAP, RANK ligand, and Runx2/Cbfa1 levels significantly correlated with aortic stenosis severity.
- mRNA levels of TRAP, RANK ligand, and RANK were significantly elevated in calcified valve areas.
- Local TRAP transcript levels correlated with early-stage stenosis, while Runx2/Cbfa1 predicted advanced-stage severity.
Conclusions:
- Bone turnover plays a significant role in determining the severity of aortic stenosis.
- Specific bone remodeling markers are associated with different stages of aortic valve disease progression.
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