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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...

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Updated: May 13, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
09:51

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A practical guide to the ICPL_ESIQuant software for ICPL-based quantitative proteomics.

Achim Brunner1, Josef Kellermann, Friedrich Lottspeich

  • 1Max-Planck-Institute of Biochemistry, Martinsried, Germany.

Biochimica Et Biophysica Acta
|March 5, 2013
PubMed
Summary
This summary is machine-generated.

ICPL_ESIQuant is a free proteomics software for analyzing mass spectrometric data from ICPL experiments. It quantifies labeled proteins and provides detailed peptide and protein information for various experimental setups.

Keywords:
CVICPLIsotope-coded protein labelLCMass spectrometryProtein quantification softwareQuantitative proteomicsXICcoefficient of variationextracted ion chromatogramliquid chromatography

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Area of Science:

  • Proteomics
  • Computational Biology
  • Mass Spectrometry

Background:

  • Quantitative proteomics relies on accurate analysis of complex mass spectrometry data.
  • Isotope-coded protein labeling (ICPL) is a common technique for relative protein quantification.
  • Existing software may have limitations in handling diverse data formats and experimental designs.

Purpose of the Study:

  • To introduce ICPL_ESIQuant, a novel software tool for quantitative proteomics.
  • To provide a detailed tutorial for using ICPL_ESIQuant with ICPL-based mass spectrometry datasets.
  • To enable reliable quantification of ICPL-labeled proteins from various experimental setups.

Main Methods:

  • Utilizes MS raw data in .mzXML format and Mascot search results in .dat format.
  • Implements algorithms for quantitative analysis of ICPL duplex, triplex, and quadruplex experiments.
  • Supports both shotgun and directed proteomics strategies, including mass inclusion lists.

Main Results:

  • ICPL_ESIQuant reliably quantifies ICPL-labeled proteins.
  • Provides comprehensive information on detected, sequenced, and identified features.
  • Processes mass spectrometric data from various vendors.

Conclusions:

  • ICPL_ESIQuant is a versatile and freely available software for quantitative proteomics.
  • The software facilitates robust analysis of ICPL-based mass spectrometry data.
  • It supports diverse experimental strategies, enhancing proteomics data interpretation.