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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: May 13, 2026

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Reverse genetics tools in zebrafish: a forward dive into endocrinology.

Yuya Sugano¹, Stephan C F Neuhauss

¹Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.

General and Comparative Endocrinology

|March 5, 2013

Summary

Zebrafish are increasingly used to model human diseases, benefiting from advanced genetic tools. These sophisticated transgenic and reverse genetic techniques enhance zebrafish

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Last Updated: May 13, 2026

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Published on: November 8, 2014

Production of Haploid Zebrafish Embryos by In Vitro Fertilization

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Published on: July 14, 2014

Dissection of Larval Zebrafish Gonadal Tissue

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Published on: April 26, 2017

Area of Science:

Genetics
Comparative Endocrinology
Disease Modeling

Background:

The zebrafish (Danio rerio) is a well-established genetic model organism.
Its utility in modeling human diseases has grown significantly in recent years.
Technological advancements have expanded the available genetic manipulation tools.

Purpose of the Study:

To review commonly used and novel reverse genetic and transgenic tools in zebrafish.
To highlight the impact of these tools on zebrafish as a model organism.

Main Methods:

Focus on reverse genetic tools (e.g., morpholinos, CRISPR/Cas9).
Focus on transgenic tools (e.g., BAC transgenesis, Tol2 transposon system).
Review of recent technological advancements in zebrafish genetic engineering.

Main Results:

Zebrafish possess a sophisticated toolkit of genetic manipulation methods.
Both established and emerging tools are readily available for researchers.
These tools facilitate precise genetic modifications for research.

Conclusions:

Recent advancements have significantly enhanced the genetic toolbox for zebrafish.
These developments make zebrafish an increasingly valuable model for comparative endocrinology and disease research.