Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Multiplex PCR Assays for Detection of Salmonella spp. and Cronobacter sakazakii on Stainless Steel and Sealed Concrete Surfaces Compared with FDA-BAM Reference Methods.

Journal of AOAC International·2026
Same author

Single-shot, depth-encoded multiplexed OCT for multi-spot tracking of induced transient corneal dynamics.

Biomedical optics express·2026
Same author

Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.

Journal of the American Academy of Dermatology·2026
Same author

Large Hypertransmission Defects Exhibit Choriocapillaris Flow Speed Impairment in Nonexudative Age-Related Macular Degeneration.

Ophthalmology science·2026
Same author

Analysis of pulsatile motion in the murine cornea using phase-sensitive visible-light optical coherence tomography.

Biomedical optics express·2025
Same author

Roadmap on advances in visual and physiological optics.

Journal of optics (2010)·2025

Related Experiment Video

Updated: May 13, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

High-precision, high-accuracy ultralong-range swept-source optical coherence tomography using vertical cavity surface

Ireneusz Grulkowski1, Jonathan J Liu, Benjamin Potsaid

  • 1Department of Electrical Engineering and Computer Science, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Optics Letters
|March 5, 2013
PubMed
Summary

Ultralong-range swept-source optical coherence tomography (OCT) imaging up to meters is now possible. This technology offers high precision for applications like human eye imaging and industrial measurements.

More Related Videos

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia
06:15

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Related Experiment Videos

Last Updated: May 13, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia
06:15

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Area of Science:

  • Biomedical Optics
  • Laser Technology
  • Imaging Systems

Background:

  • Optical coherence tomography (OCT) is a valuable imaging modality.
  • Extending OCT imaging range presents significant technical challenges.
  • Swept-source OCT (SS-OCT) offers potential for enhanced performance.

Purpose of the Study:

  • To demonstrate ultralong-range SS-OCT imaging.
  • To assess the feasibility of SS-OCT for ocular biometry and industrial applications.
  • To overcome challenges associated with long-range OCT.

Main Methods:

  • Utilized vertical cavity surface emitting laser (VCSEL) technology for swept-source OCT.
  • Adjusted laser parameters and employed high-speed acquisition.
  • Developed methods for imaging ranges from centimeters to meters.

Main Results:

  • Achieved ultralong-range OCT imaging from centimeters to meters with a single instrument.
  • Demonstrated in vivo human eye imaging with high precision and accuracy.
  • Performed meter-range optical fiber length measurements and multicentimeter component characterization.
  • Showcased 3D visualization for industrial imaging.

Conclusions:

  • Ultralong-range SS-OCT is feasible and versatile.
  • The technology provides precise measurements crucial for ocular biometry and industrial inspection.
  • VCSEL-based SS-OCT enables a new class of long-range imaging applications.