Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust strong coupling of single quantum emitters with plasmonic nanocavity on one-dimensional photonic crystal substrate.

Fundamental research·2026
Same author

Ultrasensitive Single-Particle Hygroscopic Growth Measurements of Regional Airport Aerosols.

Environmental science & technology·2026
Same author

Niobium Gradient Doping-Driven Microstructural Engineering for High-Performance Nickel-Rich Cathode Materials in Lithium-Ion Batteries.

ACS applied materials & interfaces·2026
Same author

Dual-state six-channel polarization multiplexing in reconfigurable metasurfaces.

Nanophotonics (Berlin, Germany)·2025
Same author

Stable Cycling of High-Voltage Ni-Rich Lithium Metal Batteries through Solvation Structure Regulation by Functional Electrolyte Co-Additives.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Anomalous optical gradient force induced by polarization-tuned antisymmetry in energy density gradient.

Nanophotonics (Berlin, Germany)·2025

Related Experiment Video

Updated: May 13, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Surface-plasmon-coupled emission microscopy with a polarization converter.

Yikai Chen1, Douguo Zhang, Lu Han

  • 1Institute of Photonics, Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.

Optics Letters
|March 5, 2013
PubMed
Summary

Surface-plasmon-coupled emission microscopy shows promise for sensitive surface imaging. A novel liquid-crystal plate method converts fluorescence polarization, improving optical resolution and signal collection for better imaging.

More Related Videos

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
08:01

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Related Experiment Videos

Last Updated: May 13, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures
08:01

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Published on: November 21, 2019

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Materials Science

Background:

  • Surface-plasmon-coupled emission (SPCE) microscopy offers high sensitivity for surface imaging.
  • A key limitation of SPCE is its donut-shaped point spread function (PSF), causing poor optical resolution and inefficient signal collection.

Purpose of the Study:

  • To address the limitations of SPCE microscopy by enhancing optical resolution and signal collection efficiency.
  • To demonstrate a method for converting the polarization state of SPCE fluorescence.

Main Methods:

  • Experimentally employed a liquid-crystal (LC) plate to modify the polarization of SPCE fluorescence.
  • Converted the radial polarization of SPCE fluorescence to linear polarization.
  • Focused the modified fluorescence using a collection lens to achieve an Airy disk-like PSF.

Main Results:

  • Successfully generated an Airy disk-like PSF with a smaller size compared to the conventional donut shape.
  • Achieved simultaneous enhancement in both lateral resolution and signal-to-noise ratio (SNR).

Conclusions:

  • The use of a liquid-crystal plate is a feasible method to overcome the resolution limitations of SPCE microscopy.
  • This technique offers a pathway to improved sensitivity and resolution in surface imaging applications.