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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Two-plasmid vector system for independently controlled expression of green and red fluorescent fusion proteins in
Anthony J Brzoska1, Neville Firth
1School of Biological Sciences, University of Sydney, Sydney, New South Wales, Australia.
Applied and Environmental Microbiology
|March 5, 2013
Abstract:
We have constructed a system for the regulated coexpression of green fluorescent protein (GFP) and red fluorescent protein (RFP) fusions in Staphylococcus aureus. It was validated by simultaneous localization of cell division proteins FtsZ and Noc and used to detect filament formation by an actin-like ParM plasmid partitioning protein in its native coccoid host.

