Related Experiment Video
Updated: May 11, 2026
Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 4, 2015
Sister-chromatid exchanges in lymphocytes from styrene-exposed boat builders
K T Kelsey1, T J Smith, S K Hammond
1Laboratory of Radiobiology, Harvard School of Public Health, Boston, MA 02115.
Abstract:
Sister-chromatid exchanges (SCE) were measured in peripheral blood lymphocytes from 40 workers in the boat-building trade. Twenty of these workers were exposed to significant amounts of styrene. The mean air concentration of styrene in the breathing zone of the boat builders was 209 mg/m3 in the 7 exposed current smokers and 230 mg/m3 in the 13 exposed non-smokers. Urinary styrene metabolites were also measured and the mean mandelic acid/creatinine ratios in the exposed, smokers was 275 mg/g, and in the exposed, non-smokers 323 mg/g. The SCE frequency in lymphocytes from the styrene-exposed group did not differ from that in the controls, although smoking significantly induced SCE in these workers.
More Related Videos
08:18Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells
Published on: September 5, 2017
10:47Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
Published on: November 3, 2017
Related Concept Videos
01:08Nucleotide Excision Repair
02:31Homologous Recombination
01:38Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...