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Related Concept Videos

Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Alveoli and Alveolar Ducts01:26

Alveoli and Alveolar Ducts

The respiratory zone of the human body, which stands in contrast to the conducting zone, comprises the structures that actively participate in the exchange of gases. The initiation of this zone is marked by the terminal bronchioles converging into respiratory bronchioles, the tiniest bronchiole classification. The respiratory bronchioles give way to the alveolar ducts that opens into a congregation of alveoli. Actively involved in gas exchange, alveoli resemble tiny sacs similar to clusters of...

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Related Experiment Video

Updated: May 13, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Potential candidate genes for alveolar hypoxia identified by transcriptome network analysis.

Bi-li Zhang1, Rong-liang Xu, Yong-wen Qin

  • 1Department of Cardiovascular Diseases, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.

Medicina (Kaunas, Lithuania)
|March 5, 2013
PubMed
Summary

Alveolar hypoxia, a condition linked to lung diseases, was studied using transcriptome networks. Key regulators like HIF-1α and PPARγ were identified, revealing potential gene targets and pathways involved in the hypoxia response.

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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Related Experiment Videos

Last Updated: May 13, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Area of Science:

  • Pulmonary Medicine
  • Molecular Biology
  • Genomics

Background:

  • Alveolar hypoxia is implicated in serious lung conditions, including chronic pulmonary hypertension and vascular remodeling.
  • Understanding the molecular mechanisms of hypoxia in the lungs is crucial for disease management.

Purpose of the Study:

  • To investigate the biological responses to alveolar hypoxia.
  • To identify potential transcriptome networks and key regulatory genes involved in lung hypoxia.

Main Methods:

  • Utilized the microarray dataset GSE11341 to construct a gene regulatory network.
  • Performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) term enrichment analyses.

Main Results:

  • Identified Hypoxia Inducible Factor 1 Alpha (HIF-1α), Peroxisome Proliferator-Activated Receptor Gamma (PPARγ), and Nuclear Factor-kappa B (NF-κB) as central regulatory nodes.
  • Revealed that HIF-1α influences potassium channels, glucose metabolism, and inflammation, while PPARγ and HIF-1α regulate cell cycle via CCND1.
  • Discovered novel transcriptional factors and target genes, including PFKL, ALDOA, and TFF3.

Conclusions:

  • Transcriptome network analysis is effective for identifying candidate genes in alveolar hypoxia.
  • KEGG and GO analyses aid in predicting the molecular mechanisms underlying hypoxia-related genes.