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A novel dynamic layer-by-layer assembled nano-scale biointerface: functionality tests with platelet adhesion and
Melanie G Watson1, Juan M Lopez, Mihaela Paun
1Department of Biomedical Engineering, LeTourneau University, Longview, TX, 75607, USA, melaniewatson@letu.edu.
Journal of Thrombosis and Thrombolysis
|March 5, 2013
Summary
A novel dynamic layer-by-layer (d-LbL) biointerface effectively promotes platelet adhesion and aggregation, especially under high shear conditions when activated with adenosine diphosphate (ADP). This advancement holds potential for developing improved anti-coagulation surfaces in clinical applications.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Biomedical Engineering
Background:
- Platelet adhesion and aggregation are critical in thrombosis and hemostasis.
- Developing biointerfaces that control platelet interactions is essential for medical devices and therapies.
- Existing surfaces may not adequately mimic physiological conditions for platelet activation.
Purpose of the Study:
- To develop and characterize a dynamic layer-by-layer (d-LbL) self-assembly surface as a biologically-active substrate.
- To evaluate the functionality of the d-LbL biointerface for platelet adhesion and aggregation using platelet-rich plasma (PRP) with and without adenosine diphosphate (ADP).
- To assess the potential clinical applications of this improved biointerface, particularly for anti-coagulation surfaces.
Main Methods:
- Fabrication of d-LbL surfaces on glass slides using fibrinogen, Poly (sodium 4-styrene-sulfonate), and Poly (diallydimethlyammonium chloride).
- Exposure of d-LbL surfaces to PRP with and without 20 μM ADP under static and flow (high shear) conditions.
- Characterization using phase contrast microscopy (PCM), fluorescence microscopy (FM), atomic force microscopy (AFM), and field emission-scanning electron microscopy (FE-SEM).
Main Results:
- The d-LbL surface demonstrated increased platelet adhesions and aggregation in the presence of ADP compared to plain PRP.
- High-shear conditions significantly enhanced platelet aggregation on the d-LbL surface when ADP was added.
- AFM and FE-SEM revealed distinct surface morphology and increased platelet adhesion characteristics with PRP + ADP.
Conclusions:
- The developed d-LbL biointerface is a functional substrate for studying platelet interactions.
- The d-LbL surface effectively promotes platelet aggregation, particularly under high shear stress in the presence of ADP.
- This research validates the improved biointerface for potential applications in anti-coagulation technologies.
