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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 13, 2026

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

Published on: October 30, 2012

High-throughput cloning and expression library creation for functional proteomics.

Fernanda Festa¹, Jason Steel, Xiaofang Bian

¹Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287-6401, USA.

|March 5, 2013

Summary

This summary is machine-generated.

High-throughput cloning systems accelerate protein functional characterization. These methods enable faster analysis of newly identified proteins using proteomics platforms.

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Last Updated: May 13, 2026

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning

Published on: October 30, 2012

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Molecular Biology
Proteomics

Background:

Protein function studies often rely on cloned genes for expression and assays.
Genomics has identified numerous proteins, necessitating rapid functional characterization methods.
Traditional single-gene experiments are insufficient for large-scale protein analysis.

Purpose of the Study:

To provide background on DNA cloning techniques.
To discuss and compare major high-throughput cloning systems.
To present an example of high-throughput cloning in functional proteomics.

Main Methods:

Review of DNA cloning principles.
Comparative analysis of Gateway® Technology, Flexi® Vector Systems, and Creator(TM) DNA Cloning System.
Demonstration of a high-throughput cloning workflow.

Main Results:

High-throughput cloning systems offer speed, flexibility, and reliability.
These systems facilitate coupling with proteomics platforms like microarrays and cell-based assays.
An example study showcases the application in functional proteomics.

Conclusions:

Fast, flexible, and reliable cloning systems are crucial for modern proteomics.
High-throughput cloning accelerates the functional characterization of novel proteins.
This approach integrates genomics with functional proteomics for biological discovery.