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Related Experiment Video

Updated: May 13, 2026

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Published on: March 22, 2017

Imaging retinal progenitor lineages in developing zebrafish embryos.

Patricia Jusuf, William A Harris, Lucia Poggi

    Cold Spring Harbor Protocols
    |March 5, 2013
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details creating and analyzing four-dimensional (4D) movies of zebrafish retinal development in vivo. These 4D movies enable precise reconstruction of retinal cell lineages by tracking cell birth and division over time.

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    Published on: July 19, 2010

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    Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos

    Published on: July 19, 2010

    Area of Science:

    • Developmental Biology
    • Neuroscience
    • Cell Biology

    Background:

    • Understanding retinal cell development is crucial for regenerative medicine and treating vision disorders.
    • In vivo lineage tracing provides insights into cell fate determination and differentiation processes.
    • Zebrafish embryos offer a powerful model system for studying vertebrate development due to their optical transparency and rapid development.

    Purpose of the Study:

    • To describe a protocol for generating and analyzing four-dimensional (4D) movies of retinal lineage in zebrafish embryos.
    • To enable detailed in vivo tracking of individual retinal progenitor cells through multiple cell divisions, migration, and differentiation.
    • To facilitate the reconstruction of complete retinal cell lineages.

    Main Methods:

    • Utilizing transgenic zebrafish expressing fluorescent reporters in specific retinal progenitor cells.
    • Performing long-term, live imaging using confocal microscopy to capture 3D spatial data over time (4D).
    • Maintaining cell health and developmental conditions throughout extended imaging periods (up to ~50 hours).

    Main Results:

    • Successful generation of 4D movies capturing dynamic retinal development in vivo.
    • Ability to track individual cells, identify their birth timing, and determine sister cell relationships.
    • Reconstruction of detailed retinal lineages based on observed cell behaviors.

    Conclusions:

    • This protocol provides a robust method for in vivo retinal lineage tracing in zebrafish.
    • The generated 4D movies offer unprecedented insights into the dynamics of retinal cell development.
    • This technique is valuable for studying cell fate, differentiation, and the origins of retinal cell types.