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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

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Related Experiment Video

Updated: May 13, 2026

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Published on: January 13, 2023

OpenCFU, a new free and open-source software to count cell colonies and other circular objects.

Quentin Geissmann¹

¹Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom. qgeissmann@gmail.com

|March 5, 2013

Summary

Manually counting cell colonies is time-consuming. OpenCFU is a new, robust, and accurate open-source software for automatically enumerating circular objects in images and videos, improving biological research efficiency.

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Area of Science:

Biology
Bio-imaging
Computational Biology

Background:

Accurate enumeration of circular objects like cell colonies is crucial for biological research.
Manual counting methods are often subjective, slow, and labor-intensive.

Purpose of the Study:

To develop and present OpenCFU, a novel software tool for automated enumeration of circular objects.
To provide a robust, accurate, and user-friendly solution for image-based cell colony counting.

Main Methods:

Development of a cross-platform, open-source software program named OpenCFU.
Implementation of an intuitive graphical user interface for parameter control.
Testing the software on digital pictures and video streams containing circular objects.

Main Results:

OpenCFU demonstrated high robustness, accuracy, and speed in enumerating circular objects.
The software offers user control over processing parameters.
It features a modern and intuitive interface for ease of use.

Conclusions:

OpenCFU provides an efficient and reliable alternative to manual cell colony counting.
The open-source nature and cross-platform compatibility enhance its accessibility for researchers.
This tool can significantly improve the speed and objectivity of quantitative biological image analysis.