[Nonribosomal peptides synthetases gene clusters and core domain in Pseudoalteromonas sp. NJ631]

We studied nonribosomal peptides synthetases (NRPSs) gene clusters and the core module of NRPSs in Pseudoalteromonas sp. NJ631 using genome mining approach.

The genome of Pseudoalteromonas sp. NJ631 was constructed by the next genome sequencing (NGS) technology. We adopted an online available software called NRPS-PKS knowledgebase to identify potential NRPSs gene clusters within genes involved in the biosynthesis of secondary metabolite of Pseudoalteromonas sp. NJ631. The genes encoding adenylation (A) domains, the core module of NRPSs, were collected and analyzed using genome mining method.

We identified three typical NRPS gene clusters comprising three ORFs which encode six continuous modular NRPSs. The result of genome mining indicates that genome of Pseudoalteromonas sp. NJ631 contains 38 A domain genes which show 60% similarity below to their closest relatives. The substrate of these A domains was predicted to specifically bind 18 types of amino acids using the specificity-conferring selection rule.

This is the first reported on the systematic screening and analysis of NRPSs gene clusters and A domains in genus Pseudoalteromonas, suggesting that the genus Pseudoalteromonas possesses a vast array of secondary metabolite biosynthesis genes that were previously found mostly in actinomycetes and fungi. The information on secondary metabolite genes from Pseudoalteromonas sp. NJ631 will facilitate us to isolate novel nonribosomal peptides.