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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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Related Experiment Video

Updated: May 13, 2026

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Sequence Analysis of Native Oligosaccharides Using Negative ESI Tandem MS.

Zhenqing Zhang1, Robert J Linhardt

  • 1Departments of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Current Analytical Chemistry
|March 6, 2013
PubMed
Summary

Mass spectrometry, particularly electrospray ionization tandem MS, simplifies complex carbohydrate analysis. This technique enables routine sequencing of various oligosaccharides and polymers, overcoming previous analytical hurdles.

Keywords:
ESI tandem MSGlycosaminoglycan oligosaccharidesNeutral oligosaccharideSequenceUronic acid oligosaccharides

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Glycobiology

Background:

  • Carbohydrates possess crucial physiological and pharmacological activities.
  • Their complex structures present significant analytical challenges.
  • Mass spectrometry (MS) has emerged as a powerful tool for carbohydrate analysis.

Purpose of the Study:

  • To review the application of electrospray ionization tandem MS for oligosaccharide sequence analysis.
  • To highlight the utility of ESI-MS for complex carbohydrate structures.
  • To discuss the analysis of neutral saccharides, uronic acid polymers, and epimer-containing structures.

Main Methods:

  • Utilizing soft ionization techniques, specifically electrospray ionization (ESI).
  • Employing tandem mass spectrometry (MS/MS) for structural elucidation.
  • Focusing on the analysis of native oligosaccharides and complex carbohydrate polymers.

Main Results:

  • ESI tandem MS facilitates the ionization and vaporization of large, polar, and labile biomolecules.
  • This methodology has made carbohydrate analysis more routine.
  • The technique is effective for sequencing peptides, proteins, lipids, nucleic acids, and carbohydrates.

Conclusions:

  • ESI tandem MS is a vital tool for the sequence analysis of native oligosaccharides.
  • The method addresses challenges in analyzing neutral saccharides with multiple linkages.
  • It is applicable to uronic acid polymers like alginate and glycosaminoglycans with epimers.