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Vascular endothelial cells migrate centripetally within embryonic arteries
B Christ1, R E Poelmann, M M Mentink
1Department of Developmental Biology, Ruhr-University Bochum, Federal Republic of Germany.
Anatomy and Embryology
|January 1, 1990
Summary
Quail endothelial cells migrate into chick blood vessels, reaching the aorta. This vascular endothelial cell migration was halted by a synthetic peptide mimicking fibronectin, indicating active cell movement.
Area of Science:
- Developmental biology
- Vascular biology
- Cell migration
Background:
- Endothelial cells form blood vessels and play roles in development and disease.
- Understanding endothelial cell migration is crucial for tissue repair and tumor growth.
Purpose of the Study:
- To investigate the migratory behavior of vascular endothelial cells in a developing organism.
- To determine if endothelial cells actively migrate and contribute to host vasculature.
Main Methods:
- Quail-chick chimera model using heterospecific transplantation of vascularized tissues.
- Immunohistochemical staining to identify and track quail endothelial cells within chick hosts.
- Inhibition of cell migration using a synthetic peptide containing the RGD-sequence.
Main Results:
- Quail endothelial cells successfully invaded chick host vessels, with a preference for arteries.
- Migrating endothelial cells were observed to reach the host aorta and contribute to its endothelium.
- Microinjection of an RGD-sequence peptide significantly inhibited endothelial cell invasion.
Conclusions:
- Endothelial cells exhibit active migratory behavior.
- The RGD-sequence, mimicking fibronectin, plays a role in mediating endothelial cell migration.
- These findings provide insights into the mechanisms of vascular development and cell trafficking.