Metal uptake and nanoparticle synthesis in hairy root cultures

: Hairy roots are a convenient experimental tool for investigating the interactions between plant cells and metal ions. Hairy roots of species capable of hyperaccumulating Cd and Ni have been applied to investigate heavy metal tolerance in plants; hairy roots of nonhyperaccumulator species have also been employed in metal uptake studies. Furnace treatment of hairy root biomass containing high concentrations of Ni has been used to generate Ni-rich bio-ore suitable for metal recovery in phytomining applications. Hairy roots also have potential for biological synthesis of quantum dot nanocrystals. As plant cells intrinsically provide the confined spaces needed to limit the size of nanocrystals, hairy roots cultured in bioreactors under controlled conditions are a promising vehicle for the manufacture of peptide-capped semiconductor quantum dots.