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Changes in membrane fatty acid composition of Pseudomonas aeruginosa in response to UV-C radiations
Salma Kloula Ben Ghorbal1, Abdelwaheb Chatti, Mohamed Marwan Sethom
1Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux CERTE, Technopole Borj Cédria, P.O. Box 273, 8020 Soliman, Tunisia.
Abstract:
The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C.
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