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Related Experiment Video

Updated: May 13, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

NIR-emitting quantum dot-encoded microbeads through membrane emulsification for multiplexed immunoassays.

Xiebing Wang1, Gang Wang, Wanwan Li

  • 1State Key Lab of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China.

Small (Weinheim an Der Bergstrasse, Germany)
|March 7, 2013
PubMed
Summary

This study introduces novel near-infrared quantum dot (QD)-encoded microbeads for multiplexed detection of hepatitis B virus (HBV) using flow cytometry. This method enables simultaneous detection of multiple hepatitis B virus biomarkers.

Keywords:
SPG membrane emulsificationimmunoassaysmicrobeadsmultiplexed detectionquantum dots

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Nanotechnology and Materials Science
  • Infectious Disease Diagnostics

Background:

  • Accurate and multiplexed detection of hepatitis B virus (HBV) biomarkers is crucial for effective diagnosis and management.
  • Existing diagnostic methods may lack the sensitivity or multiplexing capability required for comprehensive HBV profiling.
  • Quantum dots (QDs) offer unique optical properties suitable for advanced biosensing applications.

Purpose of the Study:

  • To develop near-infrared (NIR) emitting CdSeTe/CdS/ZnS core/shell/shell QD-encoded microbeads for multiplexed HBV detection.
  • To establish a facile synthesis route for stable, highly fluorescent QDs and their incorporation into microbeads.
  • To demonstrate the application of these QD-encoded microbeads in a flow cytometry-based multiplex immunoassay for HBV antigens and antibodies.

Main Methods:

  • A one-pot synthesis was employed to create CdSeTe/CdS/ZnS core/shell/shell QDs with high photoluminescence quantum yield and stability.
  • Shirasu porous glass (SPG) membrane emulsification was used to encapsulate QDs into polystyrene-maleic anhydride (PSMA) microbeads.
  • A 'single wavelength' encoding strategy utilizing varying emission intensity and wavelength of NIR QDs was developed for multiplexing.
  • Flow cytometry (Beckman Coulter FC 500 with a 488 nm laser) was utilized for the detection assays.

Main Results:

  • Highly fluorescent and stable NIR-emitting QD-encoded microbeads were successfully prepared.
  • A multiplexed assay demonstrated the capability to detect hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in a 2-plex format.
  • The system also successfully performed a 3-plex assay for hepatitis B surface antibody (HBsAb), hepatitis B e antibody (HBeAb), and hepatitis B core antibody (HBcAb).

Conclusions:

  • NIR-emitting QD-encoded microbeads offer a promising platform for sensitive and multiplexed detection of HBV biomarkers.
  • The developed method integrates facile QD synthesis, robust microbead encoding, and standard flow cytometry for efficient immunoassays.
  • This technology holds potential for advancing diagnostic tools for hepatitis B virus infection.