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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Updated: May 13, 2026

RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells

RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells

Published on: June 16, 2023

Microarray expression profile of long noncoding RNAs in human osteosarcoma.

Jin-Ping Li¹, Li-Hong Liu, Jie Li

¹Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha 410010, PR China.

Biochemical and Biophysical Research Communications

|March 8, 2013

Summary

This summary is machine-generated.

This study identifies differentially expressed long noncoding RNAs (lncRNAs) in osteosarcoma. These findings suggest lncRNAs could serve as novel diagnostic biomarkers and therapeutic targets for osteosarcoma.

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Area of Science:

Genomics
Molecular Biology
Oncology

Background:

Long noncoding RNAs (lncRNAs) play a crucial role in genome regulation.
Altered lncRNA expression is implicated in various cancers, but their role in osteosarcoma is unexplored.

Purpose of the Study:

To investigate the expression profile of lncRNAs in osteosarcoma.
To identify potential lncRNA biomarkers and therapeutic targets for osteosarcoma.

Main Methods:

Microarray analysis to compare lncRNA expression in osteosarcoma versus adjacent noncancerous tissues.
Quantitative real-time polymerase chain reaction (PCR) for lncRNA validation.
Bioinformatic analyses including gene ontology, pathway, and network analysis.

Main Results:

Identified 25,733 expressed lncRNAs in osteosarcoma.
Found 403 consistently over-regulated and 798 consistently under-regulated lncRNAs.
Pathway analysis revealed significant differences in 32 under-regulated and 34 over-regulated pathways.

Conclusions:

This is the first study to reveal differentially expressed lncRNAs in osteosarcoma.
lncRNAs represent promising novel candidate biomarkers for osteosarcoma diagnosis.
lncRNAs may offer potential therapeutic targets for osteosarcoma treatment.