Lysozyme immobilization via adsorption process using sulphonic acid functionalized silane grafted copolymer

Area of Science:

• Materials Science
• Biochemistry
• Chemical Engineering

Background:

• Development of efficient adsorbents is crucial for protein purification.
• Silane-based materials offer tunable properties for adsorption applications.

Purpose of the Study:

• Synthesize and characterize a novel silane-based adsorbent, (SA-g-E)-g-APST/SO3H.
• Investigate the adsorption behavior of Lysozyme (LYZ) protein onto the synthesized material.
• Evaluate the adsorbent's performance for protein immobilization and regeneration.

Main Methods:

• Synthesis and characterization of the adsorbent using FTIR, XRD, and TGA.
• Batch adsorption experiments to study protein-particle interaction.
• Kinetic and isotherm modeling (Pseudo-second-order, Sips).
• Thermodynamic studies and regeneration experiments.

Main Results:

• The synthesized adsorbent [(SA-g-E)-g-APST/SO3H] was successfully characterized.
• Optimal pH for Lysozyme adsorption was 7.0, with equilibrium reached in 3 hours.
• The Sips isotherm model best described the adsorption, yielding a maximum capacity of 37.68 mg/g.
• Adsorption was found to be exothermic, spontaneous, and the adsorbent demonstrated good reusability over six cycles.

Conclusions:

• The sulfonated silane-based adsorbent [(SA-g-E)-g-APST/SO3H] is effective for Lysozyme immobilization.
• The material exhibits favorable adsorption kinetics, high capacity, and excellent regeneration capability.
• This adsorbent shows promise for applications in protein purification and separation technologies.