Related Experiment Video
Updated: May 13, 2026

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha
Eva Eilert1, Andreas Kranz, Cornelis P Hollenberg
1ARTES Biotechnology GmbH, Elisabeth-Selbert-Str. 9, 40764 Langenfeld-Rheinland, Germany. eva.eilert@uni-duesseldorf.de
Abstract:
Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. Hansenula polymorpha was engineered to catalyze the transformation of monomeric substrates to 5-hydroxyectoine. For this purpose four genes encoding the enzymes of the 5-hydroxyectoine biosynthesis pathway of Halomonas elongata, EctA, EctB, EctC, and EctD, were inserted into the genome of H. polymorpha. Subsequently the syntheses of ectoine and 5-hydroxyectoine were analyzed and optimized. We showed that H. polymorpha is a suitable system for recombinant 5-hydroxyectoine synthesis in gram per liter scale (2.8 g L⁻¹ culture supernatant, 365 μmol/g dcw) in which almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity were achieved.
More Related Videos
05:44Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
Published on: December 27, 2024
13:16Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Related Concept Videos
Stringent Response in E. coli
Exocytosis
Exocytosis
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Gene Regulation During Sporulation
Yeast Signaling