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Published on: June 15, 2019
Correlation between hyaluronic acid,hyaluronic Acid synthase and human renal clear cell carcinoma
Jian-Liang Cai1, Ming Li, Yan-Qun Na
1Peking University Wu Jieping Urological Center, Peking University Shougang Hospital, Beijing 100144, China.
Insights
Hyaluronic acid (HA) and hyaluronic acid synthases (HASs) may not significantly impact human renal clear cell carcinoma (RCCC) progression, despite altered mRNA and protein expression levels. Further research is needed to clarify their roles in RCCC development.
Area of Science:
- Oncology
- Biochemistry
- Molecular Biology
Background:
- Hyaluronic acid (HA) and its synthesizing enzymes, hyaluronic acid synthases (HASs), are implicated in various cancers.
- The specific roles of HA and HAS isoforms in human renal clear cell carcinoma (RCCC) remain largely unelucidated.
Purpose of the Study:
- To investigate the correlation between hyaluronic acid (HA) and hyaluronic acid synthase (HAS) expression and human renal clear cell carcinoma (RCCC).
Main Methods:
- Quantitative analysis of three HAS isoforms' mRNA and protein expression, alongside HA levels, in 93 RCCC tissues and 27 adjacent normal kidney tissues.
- Techniques employed included Real-Time RT-PCR, Western Blot, and immunohistochemistry.
Main Results:
- All HAS isoforms' mRNA were upregulated in RCCC compared to normal tissues, with HAS3 showing higher expression at the mRNA level.
- While HAS3 protein expression was significantly increased in RCCC, overall HAS protein levels were comparable between RCCC and normal tissues.
- A significant decrease in HA expression was observed in RCCC tissues compared to adjacent normal tissues.
Conclusions:
- Hyaluronic acid (HA) and most hyaluronic acid synthases (HASs), excluding HAS3, may not play a critical role in the biological progression of human renal clear cell carcinoma (RCCC).
- The observed differential expression patterns suggest a complex regulatory mechanism of HA metabolism in RCCC.
Objective:
To study the correlation between hyaluronic acid (HA), hyaluronic acid synthase (HAS) and human renal clear cell carcinoma (RCCC).
Methods:
The expression of three HAS isoforms' gene and HA in 93 RCCC tissues, 27 nephridial tissues by the side of RCCC from two hospitals were measured with Real-Time RT-PCR、Western Blot and immunohistochemical methods and analyzed.
Results:
All RCCC and adjacent normal tissues expressed three HASs' mRNA & protein; at the mRNA level, both RCCC and adjacent normal tissues, expressed more HAS3 than HAS1 or HAS2, their differences were statistically significant (all P values <0.05); but, at the protein level, all HAS isoforms presented the equivalent expression. Compared with the adjacent non-neoplastic kidney tissues, the expression of all HAS isoforms' mRNA in RCCC tissues were increased evidently and their differences were significant (all P values <0.0001); but at the protein level, only the expression of HAS3 increased evidently (P=0.022). In all adjacent normal tissues, more than 80% renal tubular cells strongly expressed HA, however, only the minority RCCC cases (16/93) presented weakly positive HA staining in few cancer nests (5%-30%), the difference were significant (P<0.0001). In RCCC tissues subgrouped according to clinical stage, pathological grade, lymphatic metastasis or not and distant metastasis or not, the HASs' mRNA & protein differential expression all had no statistical significance (all P values >0.05).
Conclusion:
Different from other malignancy, HA and HASs (except for HAS3) may not play important roles in the biological progress of human RCCC.
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