[ATP synthesis by myosin during pH-jump]

It has been shown that a fast increase in pH of myosin solution leads to ATP formation de novo from ADP and Pi (about one ATP molecule per one myosin molecule). The obligatory condition for ATP synthesis was that the value of pH 7.9-8.0 should be crossed in the course of pH-jump. The data obtained are explained in terms of the concept of conformational relaxation in enzyme catalysis.