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Updated: May 13, 2026

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
Published on: May 18, 2022
Long-term, inducible gene loss-of-function in the chicken embryo
Olivier Serralbo1, Cyril A Picard, Christophe Marcelle
1EMBL Australia, Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton, Victoria, 3800, Australia. olivier.serralbo@monash.edu
Abstract:
The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development.

