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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
The hepatitis E virus capsid C-terminal region is essential for the viral life cycle: implication for viral genome
Tomoyuki Shiota1, Tian-Cheng Li, Sayaka Yoshizaki
1Department of Virology II, National Institute of Infectious Diseases, Gakuen, Musashi-murayama, Tokyo, Japan.
Abstract:
Although the C-terminal 52 amino acids (C52aa) of hepatitis E virus (HEV) capsid are not essential for morphology, the C52aa-encoding region is required for replication. Transfection of a C52aa knockdown mutant showed transient growth, and the earliest population included a majority of noninfectious (possibly empty) particles and a minority of infectious particles with C-terminal capsid degradation. Finally, the complete revertant was generated reproducibly. C52aa is essential for the viral life cycle, promoting accurate encapsidation and stabilizing encapsidated particles.
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