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Sulphinpyrazone reduces endocardial injury and mural thrombosis
1Department of Pathology, University of Auckland School of Medicine, New Zealand.
Study Objective:
The aim of the study was to investigate the effectiveness of sulphinpyrazone, a drug which stabilises endothelial cell membranes, in reducing endocardial injury and mural thrombosis produced by lactic acid in the left ventricle.
Design:
The left ventricular endocardium of isolated beating rat hearts, perfused via the aorta with oxygenated Krebs-Henseleit buffer, was exposed for up to 4 h to additional lactic acid (pH 6.4), with and without sulphinpyrazone (100 ng x ml-1). After flushing with buffer, passage of 10 ml blood, and further flushing, the hearts were fixed by coronary perfusion and the endocardium examined by scanning electron microscopy.
Experimental Material:
Hearts from 48 male albino Wistar rats, weight 270-380 g, were used.
Main Results:
Morphometric analysis of the surface of the papillary muscles showed that lactic acid caused membrane injury in endothelial cells, up to 30% of which exfoliated. However when sulphinpyrazone was present, endothelial cell damage was reduced and there was up to 75% reduction in the area of exposed basal lamina or connective tissue. This was associated with a corresponding reduction in the extent of platelet adhesion (79%) and thrombus formation (94%).
Conclusions:
The results show that sulphinpyrazone has the potential to reduce the risk of mural thrombosis following endocardial injury.